An Integrated Approach of QTL Mapping and Genome-Wide Association Analysis Identifies Candidate Genes for Phytophthora Blight Resistance in Sesame (Sesamum indicum L.)

Phytophthora blight (PB) caused by nicotianae is a highly destructive disease in sesame ( Sesamum indicum L.). In this study, we used linkage mapping and genome-wide association study (GWAS) to identify quantitative trait loci (QTL) candidate genes associated with PB resistance. The QTL 90 RILs of the Goenbaek × Osan cross using genotyping-by-sequencing detected significant QTLs for resistance on chromosome 10, explaining 12.79%–13.34% phenotypic variation. Association locus was also revealed through bulked segregant analysis second RIL population (Goenbaek Milsung cross) comprising 188 RILs. GWAS 87 accessions evaluated against three P. isolates identified 29 SNPs 10 significantly These were located within 0.79 Mb region, which co-located intervals populations, hence scanned identifying genes. This region contained several defense-related R genes, five selected expression analysis. One these SIN_1019016 found show higher resistant parent compared that susceptible parents Paired-end sequencing gene parental cultivars two synonymous between exon 2 coding DNA sequence. results suggested as one conferring sesame. findings from will be useful marker-assisted selection well functional gene(s)